Identification of mouse colony-forming endothelial progenitor cells for postnatal neovascularization: a novel insight highlighted by new mouse colony-forming assay
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چکیده
منابع مشابه
Identification of multipotent progenitors in the embryonic mouse kidney by a novel colony-forming assay.
Renal stem or progenitor cells with a multilineage differentiation potential remain to be isolated, and the differentiation mechanism of these cell types in kidney development or regeneration processes is unknown. In an attempt to resolve this issue, we set up an in vitro culture system using NIH3T3 cells stably expressing Wnt4 (3T3Wnt4) as a feeder layer, in which a single renal progenitor in ...
متن کاملThe effect of corticotropin on mouse spleen colony-forming cells.
Several investigators have reported a retechnique. There was a marked decrease duction in the number of colony-forming in spleen CPU in these mice when cornunitsjCFU) in the sple.ns of mice exposed pared with control animals, but marrow to hypoxia and have postulated that this is CPU were not reduced. These tesults mdis.condary to a direct toxic effect of hypoxcafe that part of the effect of hy...
متن کاملColony forming cell assays for human hematopoietic progenitor cells.
Hematopoietic stem cells (HSCs) present in small numbers in adult bone marrow (BM), peripheral blood (PB) and umbilical cord blood (CB) produce a heterogeneous pool of progenitors that can be detected in vitro using colony forming cell (CFC) assays. Hematopoietic progenitor cells proliferate and differentiate to produce colonies of maturing cells when cultured in a semisolid methylcellulose-bas...
متن کاملColony Forming Unit Endothelial Cells Do not Exhibit Telomerase Alternative Splicing Variants and Activity
Introduction: Endothelial progenitor colony forming unit-endothelial cells (CFU-EC) were first believed to be the progenitors of endothelial cells, named endothelial progenitor cells. Further studies revealed that they are monocytes regulating vasculogenesis. The main hindrance of these cells for therapeutic purposes is their low frequency and limited replicative potentials. This study was unde...
متن کاملFractionation of mouse bone marrow by adherence separates primitive hematopoietic stem cells from in vitro colony-forming cells and spleen colony-forming cells.
Fractionation of mouse bone marrow by adherence to tissue culture plastic was used to characterize the adhesive properties of hematopoietic stem (HS) cells capable of long-term reconstitution. The adherent fraction that represents approximately 13% of the total marrow population was virtually devoid of in vitro colony-forming cells and spleen colony-forming cells but did contain approximately 3...
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ژورنال
عنوان ژورنال: Stem Cell Research & Therapy
سال: 2013
ISSN: 1757-6512
DOI: 10.1186/scrt168